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insulin secretion
Insulin Secretion, supplied by ALPCO, used in various techniques. Bioz Stars score: 98/100, based on 2397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/insulin+secretion/Insulin+ELISA/pm41932324-425-0-12
Average 98 stars, based on 2397 article reviews
Insulin Secretion, supplied by ALPCO, used in various techniques. Bioz Stars score: 98/100, based on 2397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/insulin+secretion/Insulin+ELISA/pm41932324-425-0-12
Average 98 stars, based on 2397 article reviews
insulin secretion - by Bioz Stars,
2026-10
98/100 stars
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Enzyme-linked Immunosorbent Assay:Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Article Title: ATM phosphorylation of CD98HC increases antiporter membrane localization and prevents chronic toxic glutamate accumulation in Ataxia telangiectasia Article Snippet: .. Article Title: Pre-adaptation of stem cell-derived islet organoids to hypoxia via zinc transportation inhibition drives angiogenesis. Article Snippet: .. Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: .. Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 10 4 cells/well) for 3 d, and mouse or human islet cells were cultured in dishes for 2 d. The cells were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgSO 4 , 1.2 mM KH 2 PO 4 , 25 mM NaHCO 3 , 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 * 104 cells/well) for 3 d, and mice or human islet cells were cultured in dishes for 2 d. They were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4, 25 mM NaHCO3, 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance. Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 104 cells/well) for 3 d, andmouseor human islet cellswere cultured indishes for 2 d. The cells were washed once with KRHB buffer (119mM NaCl, 4.8mM KCl, 2.5mMCaCl2, 1.2mMMgSO4, 1.2mMKH2PO4, 25mMNaHCO3, 10mM HEPES, 0.1% BSA) containing 2.8mM glucose and fasted in KRHB buffer with 2.8mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8mM glucose for 1 h. The supernatant was collected from each well, and Article Title: PRX-3140, a 5-HT4 Partial Agonist and Sigma-1 Agonist/Antagonist, Modulates Glucocorticoid Insulin Suppression and Cortisol Levels Article Snippet: After a final incubation for 2 hours (37°C, 5% CO 2 , 95% humidity), the supernatant was removed to measure the concentration of insulin using the ALPCO Insulin Rodent (Mouse/Rat) Chemiluminescence ELISA (ALPCO, Salem NH). .. Cell Culture:Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 10 4 cells/well) for 3 d, and mouse or human islet cells were cultured in dishes for 2 d. The cells were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgSO 4 , 1.2 mM KH 2 PO 4 , 25 mM NaHCO 3 , 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 * 104 cells/well) for 3 d, and mice or human islet cells were cultured in dishes for 2 d. They were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4, 25 mM NaHCO3, 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance. Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 104 cells/well) for 3 d, andmouseor human islet cellswere cultured indishes for 2 d. The cells were washed once with KRHB buffer (119mM NaCl, 4.8mM KCl, 2.5mMCaCl2, 1.2mMMgSO4, 1.2mMKH2PO4, 25mMNaHCO3, 10mM HEPES, 0.1% BSA) containing 2.8mM glucose and fasted in KRHB buffer with 2.8mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8mM glucose for 1 h. The supernatant was collected from each well, and Incubation:Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 10 4 cells/well) for 3 d, and mouse or human islet cells were cultured in dishes for 2 d. The cells were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgSO 4 , 1.2 mM KH 2 PO 4 , 25 mM NaHCO 3 , 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 * 104 cells/well) for 3 d, and mice or human islet cells were cultured in dishes for 2 d. They were washed once with KRHB buffer (119 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4, 25 mM NaHCO3, 10 mM HEPES, 0.1% BSA) containing 2.8 mM glucose and fasted in KRHB buffer with 2.8 mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8 mM glucose for 1 h. The supernatant was collected from each well, and Article Title: Trimethylamine N-oxide impairs β-cell function and glucose tolerance. Article Snippet: .. MIN6 cells were cultured in 24-well plates (8 × 104 cells/well) for 3 d, andmouseor human islet cellswere cultured indishes for 2 d. The cells were washed once with KRHB buffer (119mM NaCl, 4.8mM KCl, 2.5mMCaCl2, 1.2mMMgSO4, 1.2mMKH2PO4, 25mMNaHCO3, 10mM HEPES, 0.1% BSA) containing 2.8mM glucose and fasted in KRHB buffer with 2.8mM glucose for 1 h. Then, the cells were incubated in KRHB buffer with 2.8 or 16.8mM glucose for 1 h. The supernatant was collected from each well, and |